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Journal of Spectroscopy
30217 Vol.27, 000(2013)
Determination of Nanogram Quantities of Emodin in Pharmaceutical Preparations and Biofluids by Luminol-Myoglobin Chemiluminescence System
Yun Zhang[1]; Xili He[1,2]; and Zhenghua Song[1];
[1]Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry & Material Science, Northwest University, Xi’an 710069, China [2]School of Science, Xi’an Jiaotong University, Xi’an 710049, China
 Abstract:Based on the quenching effect of emodin on the luminol-myoglobin (Mb) reaction, a sensitive method for the determination of nanogram level emodin by flow injection chemiluminescence (FI-CL) is presented for the first time. It was found that the CL intensity from luminol-Mb system could be inhibited in the presence of emodin, and the decrement of CL intensity was linearly proportional to the logarithm of emodin concentration in the range of 0.5– 3 0 0 n g ⋅ m L − 1 (R = 0.9956) with the detection limit of 0 . 2 n g ⋅ m L − 1 (3σ). At a flow rate of 2 . 0 m L ⋅ m i n − 1 , a complete determination of emodin, including sampling and washing, could be accomplished in 0.5 min with the relative standard deviations (RSDs) of less than 3.5% (n = 5). The proposed method was successfully applied to the determination of emodin in pharmaceutical preparations and human serum samples. The possible CL mechanism of luminol-Mb-emodin reaction was explained.
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