Guest,您好!  [ 退出系统 ]
首页 - 文献检索 - 文献细览(期刊文献)
PLoS Genetics
1553-7390 Vol.08, 10(2012)
Deposition of Histone Variant H2A.Z within Gene Bodies Regulates Responsive Genes
Devin Coleman-Derr, Daniel Zilberman[*]
Department of Plant and Microbial Biology, University of California Berkeley, Berkeley, California, United States of America
 Abstract:The regulation of eukaryotic chromatin relies on interactions between many epigenetic factors, including histone modifications, DNA methylation, and the incorporation of histone variants. H2A.Z, one of the most conserved but enigmatic histone variants that is enriched at the transcriptional start sites of genes, has been implicated in a variety of chromosomal processes. Recently, we reported a genome-wide anticorrelation between H2A.Z and DNA methylation, an epigenetic hallmark of heterochromatin that has also been found in the bodies of active genes in plants and animals. Here, we investigate the basis of this anticorrelation using a novel h2a.z loss-of-function line in Arabidopsis thaliana. Through genome-wide bisulfite sequencing, we demonstrate that loss of H2A.Z in Arabidopsis has only a minor effect on the level or profile of DNA methylation in genes, and we propose that the global anticorrelation between DNA methylation and H2A.Z is primarily caused by the exclusion of H2A.Z from methylated DNA. RNA sequencing and genomic mapping of H2A.Z show that H2A.Z enrichment across gene bodies, rather than at the TSS, is correlated with lower transcription levels and higher measures of gene responsiveness. Loss of H2A.Z causes misregulation of many genes that are disproportionately associated with response to environmental and developmental stimuli. We propose that H2A.Z deposition in gene bodies promotes variability in levels and patterns of gene expression, and that a major function of genic DNA methylation is to exclude H2A.Z from constitutively expressed genes.
下载题录  |  原文收割  |  参考译文  |  推荐[0]  |  分享到:
新浪微博 腾讯微博  

关于我们   |   联系我们   |   意见或建议   |   检索技巧   |   在线客服
古扬科技   渝公网备:500199095-00008   渝ICP备:10200524号